hMSC Osteogenesis Induction Medium

The hMSC osteogenesis induction medium is a sterile liquid culture medium for inducing osteogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement mix components.

• Formulation is optimised for initial seeding of 6000 cells/cm² up to confluence (approx. 90%)
• Feeder-layer, matrix substrates, or other substances are not necessary
• Due to the possibility of reduced proliferative activity, we recommend to use the antibiotic supplement for freshly isolated cells only

After adding 50 ml FCS, 10 ml HEPES, and 5 ml L-Glutamine and optional 3,5 ml Antibiotics to the 500 ml hMSC osteogenesis induction medium basal the media can be stored in the dark at 4 to 8 °C for up to 4 weeks.

Note: After adding the Osteogenesis induction factor Dexamethasone, Ascorbic-Acid-2-phosphate, and ß-Glycerol-phosphate to the media, the media can be stored in the dark at 4 to 8 °C for up to 1 week. Therefore, each aliquot contains enough volume to prepare 50 ml hMSC osteogenesis induction media only.

To prepare 50 ml osteogenesis inductions media add 500 µl Dexamethasone, 500 µl Ascorbic-Acid-2-phosphate, and 360 µl ß-Glycerol-phosphate to 50 ml hMSC osteogenesis induction medium, basal (already supplemented with FCS, HEPES and L-Glutamine).

Do not use the supplemented media for longer than one week. Do not heat the medium over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and heat it. Do not freeze the medium. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.