Peptide coupling kits, HOOK™

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.

HOOK™ Peptide Coupling (Amine reactive) kits utilise the chemical heterobifunctional crosslinker EDC to couple peptides to carrier proteins. EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein.

HOOK™ Peptide Coupling (Sulfhydryl reactive) kits utilise a sulfhydryl group in the peptide. This kit exploits the chemical cross-linker sulfoSMCC to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins. The N-hydroxysuccinimide (NHS) ester in sulfoSMCC reacts with primary amines to form covalent amide bonds and the maleimide group reacts with sulfhydryl groups to form stable thioether bonds.

These kits utilise Tube-O-Reactor™, which allows the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands on time and effort.