3,3',5,5'-Tetramethylbenzidine free base
TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.
- Sensitive HRP Substrate: Enables high-sensitivity detection in enzyme-linked assays
- Colorimetric Detection: Produces a strong blue signal, with a yellow endpoint upon acid stop for dual-wavelength measurement
- Flexible Formulation: Free base form allows custom preparation for specific assay conditions
- Versatile Applications: Ideal for ELISA, Western blotting, and immunoassays in diagnostics and research
- Low Background: Provides high signal-to-noise ratio for accurate quantification
TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.
- Sensitive HRP Substrate: Enables high-sensitivity detection in enzyme-linked assays
- Colorimetric Detection: Produces a strong blue signal, with a yellow endpoint upon acid stop for dual-wavelength measurement
- Flexible Formulation: Free base form allows custom preparation for specific assay conditions
- Versatile Applications: Ideal for ELISA, Western blotting, and immunoassays in diagnostics and research
- Low Background: Provides high signal-to-noise ratio for accurate quantification
Product Family Options
- Pack type
Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
#: 83640.320
: VWR Chemicals
Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution
#: 31770.294
: VWR Chemicals
Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
#: 83645.320
: VWR Chemicals