A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

• 100 mM Tris.HCl, 1,5 M NaCl, 0,5% Tween-20, pH 7,5

TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.