SMAD1 (pS463/S465 + Total SMAD1) ELISA Kit is a single-wash 90-min Simplestep used to quantify SMAD1 (pS463/S465 + Total SMAD1) with a sensitivity of 20 µg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader.