Human beta Nerve Growth Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human beta Nerve Growth Factor with a sensitivity of 6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Cited in over 5 citations.