Mesenchymal Stem Cells, Dental Pulp

Dental Pulp Stem Cells (DPSCs) are primarily sourced from the pulp tissues of deciduous teeth, primary incisors, and permanent third molars. The dental pulp, especially from third molars, is noted for its rich reservoir of stem cells. DPSCs derived from third molars express key pluripotency-associated transcription factors including oct4, sox2, klf4, nanog, and c-myc.

• Purity >90% by flow cytometry, Test negative for Hepatitis B and C, HIV 1/2, HTLV-I/II, HIV-1/HCV/HBV, Cryopreserved in CryoStor® CS10 media

Flow cytometry analysis confirms DPSCs are positive for mesenchymal markers CD73, CD90, CD105, and CD166, while negative for hematopoietic markers CD34, CD45, and CD133. DPSCs exhibit versatile differentiation potential under specific culture conditions, capable of forming osteogenic, adipogenic, and neurogenic cell types, and demonstrating angiogenic activity in matrigel assays.

Cells were collected from from a healthy human donors using permission forms and procedures that have been approved by the Institutional Review Board (IRB). Storage: For prolonged cell preservation or resuscitation, it is recommended to prepare the cells for extended storage in the vapor phase of liquid nitrogen. Storage of cells in the liquid phase of nitrogen is strongly discouraged to prevent potential cross-contamination risks when removing from storage. Long-term storage under −175 °C and for short-term storage, about one or two weeks at −80 °C is permitted, but it is recommended to minimise this duration to ensure optimal stability. Thawed samples should be used immediately and not stored.