Endothelial Cell Proliferations Medium

Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components.

• Formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%)
• Feeder-layer, matrix substrates, or other substances are not necessary

Due to the possibility of reduced proliferative activity, we recommend to use the antibiotic supplement for freshly isolated cells only. All components undergo rigorous testing in a stringent biological assay. Each batch is specifically examined for the proliferating characteristics of human hMSC chondrogenesis. The cells cultured in the hMSC chondrogenesis induction medium are thoroughly assessed for their morphology, adherence rate, colony-forming efficiency, and population doubling time.

Storge: After adding 10 ml HEPES and optional 3,5 ml antibiotics to the hMSC Chondrogenesis Induction Medium basal, it can be stored in the dark at 4 to 8 °C for up to 4 weeks. Once the induction supplement components 1, 2, and 3 are added, the medium can be stored for up to 1 week under the same conditions. Each aliquot is designed for preparing 50ml of hMSC Chondrogenesis Induction Medium.

To prepare 50 ml of the induction medium, add 500 µl each of ITS, Dexamethasone, Sodium pyruvate, Ascorbic-acid-2-phosphate, and Proline to 50 ml of the basal medium (already supplemented with HEPES). Always add 500 µl of TGF-ß-3 freshly (as per recommended application). Avoid using the supplemented media for more than one week (without TGF-ß-3). Do not exceed 37 °C during heating or use uncontrollable heat sources such as microwaves. When using only a portion of the medium, extract the required amount, and heat it accordingly.