The rGRFT is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Griffithsia sp. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rGRFT-LecBeads (1 ml gel) shows a binding capacity of about 6 mg or more bovine Ribonuclease B [RNase B] through its binding to a terminal [Manα(1-2)Man] glycan epitope on RNase B.

• Preparation : Recombinant GRFT is covalently coupled with agarose gel
• Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
• Protein concentration : about 10 mg/ml gel
• Binding capacity : >6 mg of glycoprotein (bovine RNase B) /ml gel
• Preservative : 0.02% [w/v] Sodium azide
• Stabilizer : None
• Store at 2-10°C. (Avoid freezing).