Specifications
- Antibody type:Primary
- Antigen name:keratin 76
- Antigen symbol:KRT76
- Clonality:Monoclonal
- Clone:KRTH/1576R
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:Q01546
- Antigen synonyms:KRT76
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
- Purification:Protein A affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
This mAb recognises basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Western blot: 0,5 - 1 µg/ml Immunohistochemistry (FFPE): 0,25-0,5 µg/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: KRT76
Clonality: Monoclonal
Clone: KRTH/1576R
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG, k
Reactivity: Human