Specifications
- Pk:0,05 mg
- Protein/peptide type:Recombinant
- Source:Cells
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimise freeze-thaw cycles.
- Endotoxin content:<0,1 ng/µg (1 IEU/µg) as determined by LAL test
- Protein synonyms:F9|F9/Coagulation factor IX/Christmas factor/Plasma thromboplastin component/PTC/Coagulation factor IXa light chain/Coagulation factor IXa heavy chain
- Protein/peptide name:Coagulation factor IX
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:49,8 kDa
- Sequence:Thr29-Thr461
- Formulation:Supplied as a 0,2 µm filtered solution of 20 mM Tris HCl, 150 mM NaCl, 10% Glycerol, pH 8,0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the Lyophilised protein in ddH₂O. Please aliquot the reconstituted solution to minimise freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
Coagulation factor IX（F9), is a member of the peptidase S1 family. It contains two EGF-like domains, a Gla domain and a peptidase S1 domain. It is primarily expressed in the liver and secreted in plasma. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa. Mutations in position 43 and 46 prevents cleavage of the propeptide, mutation in position 93 probably fails to bind to cell membranes, mutation in position 191 or in position 226 prevent cleavage of the activation peptide. Mutations of human F9 can result in thrombophilia and recessive X-linked hemophilia B (HEMB). An X-linked blood coagulation disorder characterized by a permanent tendency to hemorrhage, due to factor IX deficiency. It is phenotypically similar to hemophilia A, but patients present with fewer symptoms. Many patients are asymptomatic until the hemostatic system is stressed by surgery or trauma.
- Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays.