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- Pk:1 KIT
- Assay Type:Sandwich
- Primary antibody reactivity:Human
- Description:Human complement H - CFH ELISA kit
- Shipping temperature:Wet ice
Human complement H - CFH AccuSignal ELISA kit.
- Natural and recombinant human CFH
- There is no detectable cross-reactivity with other relevant proteins
- Expression system for standard: NSO
- Immunogen sequence: S860-R1231
Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32 - q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
Useful in sandwich ELISA for quantitative detection of antigen. Aliquot 0,1 ml per well of the 100, 50, 25, 12,5, 6,25, 3,12, 1,56 ng/ml human CFH standard solutions into the precoated 96-well plate. Add 0,1 ml of the sample diluent buffer into the control well (Zero well). Add 0,1 ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CFH standard solution and each sample be measured in duplicate.