This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

• Enzyme kinetic and regulation of enzyme activity
• Role of substrate concentration
• Effects of pH and temperature on the reaction rate
• Inhibition of enzyme reaction

Also required: Spectrophotometer and cuvettes or microplate reader and microplate.