Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7,2 to 7,6).

• Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells
• DPBS is used to irrigate, wash, and dilute mammalian cells
• Phosphate buffering maintains the pH in the physiological range
• Calcium and magnesium facilitate cell binding and clumping
• DPBS without these ions can be used to wash and rinse suspended cells

Inorganic Salts: Potassium Chloride [KCl]: 200,0000 mg/L; Potassium Phosphate Monobasic [KH₂PO₄]: 200,0000 mg/L; Sodium Chloride [NaCl]: 8000,0000 mg/L; Sodium Phosphate Dibasic [Na₂HPO₄]: 1150,0000 mg/L

Moisture content: <1,5%

Solubility: Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7,3 to 7,5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1 N HCl or 1 N NaOH. Sterile filter, if desired. Store liquid at 2 to 8 °C.

pH: 7,4 ±0,1
Osmolality: 279 ±15 mM/kg
White powder