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Total Aflatoxin ELISA Kit
Total Aflatoxin ELISA Kit
  ABCAAB285282-96
 :  Abcam
 :  
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Total Aflatoxin ELISA Kit
  ABCAAB285282-96
 :  Abcam
 :  

 

  • Pk:
    96 Tests
  • Assay Type:
    Competitive
  • Host:
  • Primary antibody reactivity:
  • Description:
    Total aflatoxin ELISA kit
  • Environmentally Preferable:

 

 

Competitive ELISA kit for the quantitative measurement of total aflatoxin in grain and feed samples.

  • Quantitative measurement of total aflatoxin in grain and feed samples
  • Detection limit: grain - 0,1 ppb; formula feed - 0,2 ppb; edible oil/peanut - 0,2 ppb; sauce/wheat/barley feed - 0,2 ppb; beer - 0,2 ppb; wine/soy sauce/vinegar - 0,1 ppb
  • Sensitivity: 0,02 ppb (ng/ml)
  • Sample recovery rate: grain/formula feed- 85 ±15%, peanut - 82 ±15%, edible oil - 85 ±15%, sauce/wheat/barley feed - 83 ±15%, beer - 84 ±15%, wine/soy sauce/vinegar - 87 ±15%

This total aflatoxin ELISA kit is based on the competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, and so on. The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.