Circlegrow® is designed for obtaining rapid growth of E. coli and high yields of plasmid DNA.

• Molecular biology grade
• Culture and bacterial media
• Extends plasmid production past log phase

At the end of log phase, cells growing in Circlegrow® begin to utilize a proprietary second carbon source that becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW to a density triple that achieved in other media. The slow post-log phase growth of bacteria in Circlegrow® eliminates the need for constant OD monitoring. Circlegrow® cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.

Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Circlegrow® formulations. Chosen reagents are combined in the accurate proportions according to the proprietary Circlegrow® formula and thoroughly blended for a uniform distribution.

Coli and high yields of plasmid DNA.