Simplest assay to screen/study/characterize thioredoxin 1 inhibitors.

• Rapid and sensitive
• Convenient and high-throughput adaptable
• Can be completed in less than one hour
• With inhibitor control

The human Thioredoxin 1 (Trx1) is a key enzyme that protects cells from oxidative stress by reducing disulfides and methionine sulfoxide groups in oxidized proteins. It also regulates DNA repair and apoptosis. In mammals, Trx1 is necessary for growth and development.

An aberrant elevated expression of Trx1 is known to promote lung, cervical, pancreatic, colorectal, and breast cancers. In this kit, Trx1 reduces quenched di-FITC-labeled oxidized glutathione (Trx1 substrate), to mono-FITC-labeled unquenched reduced glutathione. In the presence of a Trx1 inhibitor (control inhibitor included), the reaction is impeded resulting in lower or no signal.

All products are FOR RESEARCH USE ONLY.   PRODUCTS ARE NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  Customer is granted a limited use non-exclusive licence for its own internal research.  Any control manufacturer supplies with a Product is limited to use only to validate the relevant Product.  By using the Product, Customers agree not to attempt to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, processes, make-up or production of any of the Products or portions thereof.