VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

Supplier: VWR Chemicals

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Supplier: VWR Chemicals

About this item

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

The GC-Rich Template kit is specifically designed for difficult GC-rich sequences. Combined with TEMPase, GC buffers I and II promote excellent amplification. The kit is designed for initial testing before using one of the GC-TEMPase 2X Master Mixes.

Ready to use GC TEMPase 2× Master Mixes I and II are designed for amplification of GC-rich sequences.

Specifications

Application:
Multiplex
Format:
Enzyme with/without buffer
High fidelity:
No
Hot start:
Chemically modified
Storage conditions:
– 20 °C (long term), + 4 °C (6 months)
Amplicon size:
≤ 5kb
Processivity:
Standard
Exonuclease activity:
5’→3’
Product overhang:
3'A
Buffer composition:
4x GC Buffer I, 4 x GC Buffer II and 25 mM MgCl2
Concentration:
5 U/µl
Description:
GC-rich DNA Target Kit
TEMPase HotStart DNA Polymerase 5U/µl 500 units
Size:
1 x 0.1 ml + 3 x 1.5 ml
Units:
500
Features:
GC-rich