The Biosensis NGFR/p75^ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than four hours in urine only if used as directed.

• Complete kit with precise instructions

This optimised human p75^ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75^ECD ELISA assay available today, with a detection limit of at least 4 pg/ml, and a quantification range of 7,8 to 500 pg/ml.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a biotinylated mouse monoclonal anti-p75^ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards. A human p75^ECD positive control (QC sample) is provided to assure consistent assay performance.

This NGFR/p75^ECD ELISA kit employs an in-house recombinant human p75^ECD-Fc chimera as standard that has been validated against an externally available p75^ECD-Fc protein. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75^ECD as p75^ECD-Fc human equivalents.

The new monoclonal antibody pair yields higher OD values in urine samples, demonstrating its increased ability to detect natural p75^ECD in biological samples. This means that samples assayed with BEK-2239 will give higher urine p75^ECD levels, or that urine samples from healthy controls with previous urine p75^ECD concentrations