T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

• Stronger 3' - 5' exonuclease activity
• Active in PCR, RT and T4 DNA Ligase buffers

Applications include blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs, blunting of PCR products with 3'-dA overhangs, synthesis of labeled DNA probes by the replacement reaction, oligonucleotide-directed site-specific mutagenesis, ligation-independent cloning of PCR products.

Storage buffer: 20 mM potassium phosphate (pH 7,5), 200 mM KCl, 2 mM DTT, and 50% (v/v) glycerol.

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 37 °C.