- Tipo anticorpo:Primary
- Tipo di anticorpi:Low-affinity nerve growth factor receptor
- Simbolo dell'antigene:NGFR
- Clonalità:Monoclonale
- Clone:8J2
- Coniugazione:ATTO 488
- Citometria a flusso:Yes
- Ospite:Mouse
- ImmunoChimica:Yes
- ImmunoFluorescenza:Yes
- Isotipo:IgG2a
- Reattività:Human,Rat,Mouse
- Epitopo:Extracellular domain (amino acids 29-250)
- Formato:Purified IgG
- Forma:Liquid
- Sinomini antigene:Low affinity neurotrophin receptor p75NTR|NGF receptor|Gp80-LNGFR|p75 IC
- Numero amminoacido:29 to 250
- UniProtKB:Uniprot# P08138 TNR16_HUMAN
- Tampone di conservazione:PBS, pH 7.2-7.6, without preservatives
- Temperatura di conservazione:Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
- Temperatura di spedizione:2-8C
- Immunogeno:Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
- Applicazioni testate:Receptor Trafficking
- Purificazione:Protein A purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
- Conf:50 µG
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease.
This antibody reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.
Application Information:
Immunocytochemistry: 1-5 ug/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation. Flow Cytometry: 5-20 ug/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user
Type: Primary
Antigen: NGFR
Clonality: Monoclonal
Clone: 8J2
Conjugation: ATTO 488
Epitope: Extracellular domain (amino acids 29-250)
Host: Mouse
Isotype: IgG2a
Reactivity: Human, Mouse, Rat