Recombinant

HEK293 cells

Human

Store lyophilized TWEAK Human recombinant at −20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for limited period of time; it does not show any change after two weeks at 4 °C. The lyophilized protein remains stable until the expiry date when stored at −20 °C.

TWEAK

TNFSF12

Greater than 90% as determined by SDS-PAGE.

Add pyrogen free water to working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

HVDYKDDDDK PAGSAPKGRK TRARRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEEARINS SSPLRYNRQI GEFIVTRAGL YYLYCQVHFD EGKAVYLKLD LLVDGVLALR CLEEFSATAA SSLGPQLRLC QVSGLLALRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH.

Filtered and lyophilized from 0.5mg/ml in 20 mM Tris buffer and 50mM NaCl pH-7.5.

SDS-PAGE