Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Lyophilized Rat Leptin although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution Rat Leptin should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.
• Biological activity:
  Pegylated Rat Leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is only slightly lower than the non-pegylated antagonist but in vivo it has profound weight reducing effect (as compared to the non-pegylated leptin), resulting mainly from reduced food intake.
• Protein synonyms:
  LEP
• Protein/peptide name:
  Leptin, pegylated
• Purity:
  Greater than 99.0% as determined by(a) Analysis by Gel-Filtration.(b) Analysis by SDS-PAGE.
• Solubility:
  It is recommended to reconstitute the lyophilized pegylated Rat Leptin in sterile 0.4% NaHCO3 adjusted to pH-8.5, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Formulation:
  The protein was lyophilized from concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Tested applications:
  SDS-PAGE , HPLC , FuncS