E. coli

Human

Lyophilized Bcl-2 although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution Bcl-2 should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.

Apoptosis regulator Bcl 2

Bcl2-BH1

Greater than 95.0% as determined by RP-HPLC and analysis by SDS-PAGE

Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4C Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

SDS-PAGE , HPLC , FuncS