Specifications

• Source:
  E. coli
• Species:
  Human
• Storage conditions:
  Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution PDGF BB should be stored at 4 °C between 2-7 days and for future use below −18 °C. Please prevent freeze/thaw cycles.
• Biological activity:
  The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is 1 ng/ml, corresponding to Specific Activity of 1,000,000 IU/mg.
• Protein/peptide name:
  PDGF B
• Purity:
  Greater than 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in 100mM Acetic Acid.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu.
• Formulation:
  Lyophilized from 10mM Acetic Acid.
• Tested applications:
  SDS-PAGE