Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.05ng/ml, corresponding to Specific Activity of 20,000,000 IU/mg.
• Protein synonyms:
  APC1
• Protein/peptide name:
  TNF alpha
• Purity:
  > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
• Formulation:
  The concentrated protein solution (1mg/ml) was lyophilized from 20 mM phosphate buffer and 0.1M NaCl.
• Tested applications:
  SDS-PAGE , HPLC , FuncS