Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to specific activity of 10,000,000 units/mg.
• Protein synonyms:
  Urogastrone
• Protein/peptide name:
  EGF
• Purity:
  > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
• Formulation:
  Lyophilized from 0.2 um filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
• Tested applications:
  SDS-PAGE , HPLC , FuncS