Specifications

• Source:
  E. coli
• Species:
  Mouse
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 0.1 ng/ml.
• Protein synonyms:
  Urogastrone
• Protein/peptide name:
  EGF
• Purity:
  > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Molecular weight:
  6 kDa
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human.
• Formulation:
  The protein was lyophilized from concentrated (1mg/ml) solution containing 1x PBS pH-7.4.
• Tested applications:
  SDS-PAGE , HPLC , FuncS