This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Type: Primary
Antigen: CCNB1
Clonality: Monoclonal
Clone: V92.1
Conjugation: CF405S
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Hamster, Human, Mouse
- Catalog No:
- BNC040680-500
- BNC040680-100
- Antigen symbol:
- CCNB1
- CCNB1
- Antigen name:
- Cyclin B1
- Cyclin B1
- Antigen synonyms:
- CCNB1_HUMAN|CCNB1|CCNB|G2 Mitotic Specific Cyclin B1
- CCNB1_HUMAN|CCNB1|CCNB|G2 Mitotic Specific Cyclin B1
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Conjugation:
- CF® 405S
- CF® 405S
- Clone:
- Clone V92.1
- Clone V92.1
- Reactivity:
- Hamster, Human, Mouse
- Hamster, Human, Mouse
- Host:
- Mouse
- Mouse
- Gene ID:
- 23960
- 23960
- Isotype:
- IgG1 kappa
- IgG1 kappa
- ImmunoFluorescence:
- Yes
- Yes
- Concentration:
- 0,1 mg/ml
- 0,1 mg/ml
- Storage buffer:
- PBS/0,1% BSA/0,05% azide
- PBS/0,1% BSA/0,05% azide
: KIMBERLY CLARK
: VWR Collection
: VWR Chemicals
: VWR Chemicals
: VWR Collection
: VWR Collection
: VWR Collection
: VWR Collection