Maleimide activated horseradish peroxidase, EZ-Link™
Supplier: Thermo Fisher Scientific
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About this item
Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.
HRP has been maleimide activated using Sulfo-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated HRP presents an available maleimide group that can react with sulfhydryl-containing molecules.
Delivery: Kit contains two modification reagents allowing flexibility in the method of producing free sulfhydryls on macromolecules. 2-Mercaptoethylamine•HCl is a mild reagent for reduction of IgG and F(ab´)2 fragments. SATA is a sulfhydryl-containing modification reagent that reacts with primary amines to present protected sulfhydryl groups, which can be exposed upon treatment with hydroxylamine.
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