Membrane protein extraction kit, Pierce™ Mem-PER™ Plus
PIER89842
:
- Pk:1 KIT
- Description:Mem-PER™ Plus Membrane Protein Extraction Kit, 300 ml
- Environmentally Preferable:
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
- Produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
- Effective for extraction from cultured mammalian cells and mammalian tissues
- Suitable for downstream applications like SDS-PAGE, Western blotting, immunoprecipitation and protein assays
- Fast and simple with isolation of membrane proteins in approximately one hour
- No special equipments required, only a benchtop microcentrifuge, tubes, homogenisers, and pipettors are needed
The Mem-PER™ Plus Membrane Protein Extraction Kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian cells, as well as from hard and soft mammalian tissues.
: The kit contains a cell wash solution (225 ml), permeabilisation buffer (50 ml), and solubilisation buffer (25 ml).