Crosslink immunoprecipitation (IP) kit, Pierce™
Catalog # PIER26147
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1 KIT
- Description:Crosslink IP Kit, sufficient for 50 reactions using 10 μl of immobilised antibody support
Specifications
About this item
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
- Eliminates antibody contamination
- Immobilise 100 to 200 µg of antibody to prepare ready-made immunoprecipitation affinity resin (reusable) for many experiments
- Spin columns eliminate resin loss and provide for more efficient separation of solutions
The kit method involves capturing the IP antibody to protein A/G Agarose resin and covalently immobilising it to the support by crosslinking with disuccinmidyl suberate.
Delivery: This kit includes protein A/G plus agarose 0,55 ml; DSS crosslinker, 8×2 mg; coupling buffer (20X), 25 ml; IP lysis/wash buffer, 2×50 ml; conditioning buffer (100X), 5 ml; tris-buffered saline (20X), 25 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; control agarose resin, 2 ml; spin columns; and collection tubes.