3,3',5,5'-Tetramethylbenzidine (TMB) solution, high sensitivity ready-to-use

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result.

Recommended for ELISA (microwell) procedures, 3,3',5,5'-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3',5,5'-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

The TMB solution has similar sensitivity levels to chemiluminescent detection reagents; it can detect as little as 0.15 ng. The TMB solution provided is enough for at least 25 mini-gel sized (10 X 10 cm) blots. Store at 2-8°C. Keep the substrate out of direct sunlight.