Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

• Reaction set up can be done at room temperature
• Reduces non-specific amplification and prevents primer degradation during set up
• Zero-time reactivation due to hot start technology
• Speed of the polymerase allows short extension times
• Robust reactions, minimal optimisation needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with Phusion® Green buffer