HiTrap IgM Purification HP is a 1 ml column prepacked with Sepharose High Performance for fast and efficient purification of monoclonal IgM.

• Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture
• Prepacked with a thiophilic adsorption resin, 2-mercaptopyridine coupled to Sepharose High Performance
• Recovery of IgM is high with excellent activity retention
• The top and bottom frits are manufactured from porous polyethylene

Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.