Affinity coupling media, Epoxy-Activated Sepharose™ 6B

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Epoxy-activated Sepharose 6B is a pre-activated medium for immobilization of various ligands. Other ligands can be coupled through hydroxyl, amino or thiol groups. The medium has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. Epoxy-activated Sepharose 6B is formed by reacting Sepharose 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. Stable to all commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride. Stable in organic solvents, e.g. 50% dimethylformamide and 50% dioxane. Negligible volume variation due to changes in pH or ionic strength.