Cells

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: ProSci Inc.

Liver tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Novus Biologicals

BACH2 Overexpression Lysate (Adult Normal)

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood mononuclear cells (PBMC) are isolated from blood collected in FDA-registered collection centres, from IRB consented healthy human donors and following cGMP and cGTP collection guidelines.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: ProSci Inc.

Skin tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Novus Biologicals

p38 Overexpression Lysate (Adult Normal)

Supplier: ProSci Inc.

Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7,9, 1,5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors).

Supplier: PROVITRO

CD14⁺ T cells, obtained from mononuclear cells, are isolated through either negative or positive selection using immunogenetic cell separation techniques. These cells play a crucial role in the immune response against infections and are valuable for the investigation and diagnosis of immune reactions, particularly in infections and inflammation.

Supplier: PROVITRO

CD34⁺ stem and progenitor cells from umbilical cord blood are crucial for haematopoiesis and modulation of the immune response. These unique cells contribute to the formation of various blood cells, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: ProSci Inc.

Liver tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: PROVITRO

HFIB-d - Human Fibroblast from dermis - are obtained from human tissue, in particularly from the dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HFIB cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.