Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Peqlab peqGOLD, DNA Ladder, 1 kb
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
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VWR® EZ-Vision®, DNA Ladder, 100 bp
Supplier: VWR Chemicals
EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.
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VWR®, DNA ladder, 100 bp
Supplier: VWR Chemicals
Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.
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VWR®, Protein marker III (pre-stained), peqGOLD
Supplier: VWR Chemicals
Protein Marker III for monitoring protein separation, transfer efficiency and size determination.
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VWR® DNA Molecular Weight Marker, High-Range
Supplier: VWR Chemicals
High-Range DNA MW (molecular weight) marker offers 10 fragments ranging from 1503 to 48 502 bp. There is sufficient material for 75 to 150 assays.
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Peqlab peqGOLD, DNA Ladder Mix
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
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VWR®, DNA marker, 1 kb
Supplier: VWR Chemicals
DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.
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Western blot enhancer, SuperSignal™, Pierce
Supplier: Thermo Fisher Scientific
SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.
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Fluorescent western blot stripping buffer, Restore™
Supplier: Thermo Fisher Scientific
Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
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S-Nitrosylation western blot kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.
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Upstate® Autorad Orientation Markers for Western Blotting
Supplier: Merck
Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.
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Protein ladders (pre-stained), ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.
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DNA ladders
Supplier: Biotium
Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.
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Western blot stripping buffer, restore PLUS™
Supplier: Thermo Fisher Scientific
Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.
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Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate
Supplier: Thermo Fisher Scientific
SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.
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Peroxidase conjugate stabiliser/diluent, Guardian™
Supplier: Thermo Fisher Scientific
Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.
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Western blotting kit, ECL substrate, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
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Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)
Supplier: Thermo Fisher Scientific
NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
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Biotin random prime DNA labelling kit, North2South™
Supplier: Thermo Fisher Scientific
North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
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Pierce™ Clean-Blot™ IP Detection Kit and Reagents
Supplier: Thermo Fisher Scientific
Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.
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Horseradish peroxidase, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
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DAB substrate
Supplier: Thermo Fisher Scientific
DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
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HRP conjugates, HisProbe™
Supplier: Thermo Fisher Scientific
HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
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Lambda DNA phage, Hind III digest
Supplier: Rockland Immunochemicals
Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.
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DNA ladder, O'RangeRuler™
Supplier: Thermo Fisher Scientific
O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.
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Lambda DNA/HindIII Marker
Supplier: Thermo Fisher Scientific
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
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DNA ladders, GeneRuler™ and O'GeneRuler™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.
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DNA ladders, ultra low range, FastRuler
Supplier: Thermo Fisher Scientific
FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.
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DNA ladder, low range, MassRuler
Supplier: Thermo Fisher Scientific
MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.
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RNA ladder, high range, RiboRuler™
Supplier: Thermo Fisher Scientific
RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.