You searched for: Nucleic Acid Reagents

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

Supplier: AGILENT

Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Quantabio

These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: Quantabio

This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Supplier: AGILENT

Increases specificity and yield of primary PCR amplification products.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.