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You searched for: Electrophoresis Buffers

Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

TAE buffer, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Tris-Acetate-EDTA (TAE) buffer, 50X

Supplier: PanReac AppliChem

50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: LONZA

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP Biomedicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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Hybridisation buffers, formamide-free, HYB-LINK™

Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

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DNA gel loading buffer 6X

DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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IPG buffers

IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals

Supplier: MP Biomedicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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DIGE buffer kit

DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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Rapid-hyb buffer

Rapid-hyb buffer

Supplier: Cytiva

A major limitation of membrane hybridisation is speed. At commonly used probe concentrations (2 to 10 ng/ml), hybridisation normally takes 16 hours to detect single-copy gene sequences in mammalian DNA. Use of Rapid-hyb buffer reduces this time to 2 hours using similar probe concentrations.

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Low pH SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

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Wash Solution Concentrate, 20X

Supplier: SERACARE LIFE SCIENCES

Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: SERACARE LIFE SCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: MO BIO

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, PBS solution 25X concentrate (phosphate buffered saline)

Supplier: TREVIGEN

Washing buffer, PBS buffer solution 25X concentrate (phosphate buffered saline)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: MO BIO

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: TREVIGEN

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: TREVIGEN

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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