You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
TAE buffer, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.
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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20
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Tris-Acetate-EDTA (TAE) buffer, 50X
Supplier: PanReac AppliChem
50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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PBS, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals
Supplier: MP Biomedicals
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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DNA gel loading buffer 6X
Supplier: LONZA
This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.
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Tris-Glycine (TG) buffer,, MP Biomedicals
Supplier: MP Biomedicals
Tris-glycine buffer is used for western blotting and gel electrophoresis.
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IPG buffers
Supplier: Cytiva
IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals
Supplier: MP Biomedicals
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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SSC 20X buffer, MP Biomedicals
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:
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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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DIGE buffer kit
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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Rapid-hyb buffer
Supplier: Cytiva
A major limitation of membrane hybridisation is speed. At commonly used probe concentrations (2 to 10 ng/ml), hybridisation normally takes 16 hours to detect single-copy gene sequences in mammalian DNA. Use of Rapid-hyb buffer reduces this time to 2 hours using similar probe concentrations.
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Low pH SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.
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Wash Solution Concentrate, 20X
Supplier: SERACARE LIFE SCIENCES
Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: SERACARE LIFE SCIENCES
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: MO BIO
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, PBS solution 25X concentrate (phosphate buffered saline)
Supplier: TREVIGEN
Washing buffer, PBS buffer solution 25X concentrate (phosphate buffered saline)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: MO BIO
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: TREVIGEN
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: TREVIGEN
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)