Centrifugal Filters
Centrifugal filters are used in laboratory settings to manipulate peptides, proteins, oligonucleotides, DNA, and RNA samples. The devices successfully clean up labeling and PCR reactions, isolate specific sections, buffer exchange, clarify samples, and remove particulates. Filters with nylon membrane are ideal for prefiltration clean-up of large agarose or acrylamide gel debris prior to purification with an MWCO device. The centrifugal filters will meet concentration, purification, separation and desalting procedure requirements.
Filtrate centrifugal ultrafilter, Vivaspin®
Supplier: Sartorius
These centrifugal devices are ideal for the concentration or ultrafiltration of difficult to filter samples, even whole blood or cell lysates.
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Pierce™ Protein Concentrators
Supplier: Thermo Fisher Scientific
Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples such as proteins and nucleic acids.
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Ultrafiltration units, Vivaflow 50R
Supplier: Sartorius
For 0,1 to 1000 ml, Hydrosart® membrane. Vivaflow 50R is a ready to use and reusable laboratory crossflow cassette for applications like cell culture supernatant concentration, virus concentration and water sample concentration. The thin channel, flip flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single module with 50 cm² active membrane area typically concentrates 250 ml by a factor of 20 in less than 30 min.
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Abundant protein depletion spin columns, Top 2 and Top 12, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Abundant Protein Depletion Spin Columns are optimised to decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis.
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Glutathione spin columns, Pierce™
Supplier: Thermo Fisher Scientific
These spin columns provide a fast, easy to use format for purifying GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.
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Protein purification spin columns, Vivapure® Ion Exchange (IEX) Mini/Maxi
Supplier: Sartorius
Vivapure® Ion Exchange (IEX) spin colums are centrifugal devices. They incorporate Sartobind membrane adsorber technology as their chromatography matrix. Sartobind IEX membrane adsorbers are based on stabilised regenerated cellulose and display a microporous structure with a pore size of >3 μm, which is orders of magnitude larger than conventional chromatographic gel materials.
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Spin columns, PAGE-Optimizer™
Supplier: G-Biosciences
PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE. The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents. PAGE-Optimizer™ helps eliminate smeared lanes, distorted and/ or smiling bands and swollen lanes. PAGE-Optimizer™ has also been shown to reveal protein bands previously masked by the above interfering agents.
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PES centrifugal concentrator, Vivaspin® 100
Supplier: Sartorius
Vivaspin® 100 PES centrifugal concentrators are versatile ultrafiltration units for centrifugation-based sample concentration as well as pressure filtration applications.
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Sample concentrators, Vivaspin®
Supplier: Cytiva
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.
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Centrifuge tube filters, Costar® Spin-X®
Supplier: Corning
Spin-X® centrifuge tube filters use centrifugation to remove bacteria, particles, or cells from liquids, HPLC sample preparation and DNA removal from agarose or acrylamide gels.
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Fritted Spin Column, 3 ml
Supplier: G-Biosciences
The 3 ml spin columns are designed to hold up to 3 ml of resin, leaving enough rome for a further 4 ml of buffer. The unique design allows the columns to utilise a 15 ml centrifuge tube as a collection tube, so the columns can be centrifuged for rapid purification.
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Spin columns, Protein A HP SpinTrap™
Supplier: Cytiva
Protein A HP SpinTrap™ columns are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids.
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Corning® Spin-X® UF Concentrators
Supplier: Corning
These non sterile centrifugal concentrators offer a simple, one step procedure for concentrating or desalting proteins and other biomolecules with 90% or better recovery.
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Disposable spin columns for protein purification
Supplier: G-Biosciences
These disposable spin columns are designed for protein purification. These spin columns are uniquely designed, with a snap off end converting to a closure for the column for easy manipulation and use.
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