3,3',5,5'-Tetramethylbenzidine (TMB)

Supplier: Merck Millipore (Oncogene)

Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.

Supplier: G-Biosciences

TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.

Supplier: Thermo Fisher Scientific

3,3',5,5'-Tetramethylbenzidine (TMB) solution, standard sensitivity ready-to-use, precipitating

Supplier: Thermo Fisher Scientific

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result.

Supplier: MP Biomedicals

3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620 to 650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.

Supplier: MP Biomedicals

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results