You searched for: Protein Assays
Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Bicinchoninic acid disodium salt hydrate
Supplier: SIGMA ALDRICH MICROSCOPY
Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.
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VWR® Protein Precipitation Kit
Supplier: VWR Chemicals
The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
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VWR®, Bradford protein assay, proteomics grade
Supplier: VWR Chemicals
The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.
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Protein carbonyl colorimetric assay kit
Supplier: Cayman Chemical
The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
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Protein quantitation assay kits, CB-X™ Protein assay
Supplier: G-Biosciences
CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.
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Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay
Supplier: G-Biosciences
A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
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Protein assay kits, RED 660™
Supplier: G-Biosciences
RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.
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Protein quantitation assay kits, CB™ Protein assay
Supplier: G-Biosciences
CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.
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Bicinchoninic acid (BCA) reducing agent compatible protein Assay
Supplier: G-Biosciences
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.
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Thermal shift protein stability kits, GloMelt™
Supplier: Biotium
Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
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Bicinchoninic acid (BCA) protein assay
Supplier: G-Biosciences
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.
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PROTEOSTAT® protein aggregation assay
Supplier: ENZO LIFE SCIENCES
PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
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Micro Bicinchoninic Acid (BCA) protein assay
Supplier: G-Biosciences
The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).
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Compatible Lowry protein assay
Supplier: G-Biosciences
These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.
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Conjugate stabilisers
Supplier: Rockland Immunochemicals
Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.
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eFluxx-ID® multidrug resistance assay kit
Supplier: ENZO LIFE SCIENCES
The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.
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SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins
Supplier: ENZO LIFE SCIENCES
The only commercially available kit for isolation and enrichment of SUMOylated proteins.
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PROTEOSTAT® protein refolding and aggregation sensing kit for microplates
Supplier: ENZO LIFE SCIENCES
ProteoStat® Protein refolding and aggregation sensing kit provides a fractional factorial matrix design to facilitate screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a red-emitting aggregation-sensitive dye.
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Protein G Spin Antibody Purification Kit
Supplier: Abcam
Kit helps purify antibodies from serum, ascites and cell culture medium from various species.
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Human Thioredoxin 1 (Trx1) Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Simplest assay to screen/study/characterize thioredoxin 1 inhibitors.
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Glycogen Assay Kit
Supplier: Cayman Chemical
A simple and sensitive tool for assaying glycogen from tissue.
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PROTEOSTAT® thermal shift stability assay kit
Supplier: ENZO LIFE SCIENCES
This kit has been designed for monitoring protein stability under systematic thermal stress conditions.
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Human β-Secretase (BACE1) Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Highly Sensitive Assay for screening potential BACE1 inhibitors.
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Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences
Supplier: Tonbo Biosciences
Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
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β-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: Cayman Chemical
A sensitive tool for measuring β-HB levels in plasma, serum, or urine.
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Osteoclast inhibitory lectin MAB MO XMO
Supplier: US Biological
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
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L-Lactate Assay Kit
Supplier: Cayman Chemical
A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
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Coomassie (Bradford) protein assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
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Pierce™ Micro BCA™ Protein Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.
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Compat-Able™ Protein assay preparation reagent set
Supplier: Thermo Fisher Scientific
The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.