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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit

Supplier: AAT BIOQUEST

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit

Supplier: AAT BIOQUEST

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyse the oxidation of xanthine to uric acid.

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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.

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Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.

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ReadiUse™ NADP Regenerating Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.

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Amplite® Fluorimetric cADP-Ribose Assay Kit

Amplite® Fluorimetric cADP-Ribose Assay Kit

Supplier: AAT BIOQUEST

This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.

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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*

Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*

Supplier: AAT BIOQUEST

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Fluorimetric Calcium Quantitation Kit  *Red Fluorescence*

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Amplite® Colorimetric  Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Supplier: AAT BIOQUEST

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.

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Amplite® Colorimetric L-Aspartate (Aspartic Acid)  Assay Kit

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric DPP4 Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit

Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit

Supplier: AAT BIOQUEST

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

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MMP Red™ Substrate

MMP Red™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: AAT BIOQUEST

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Amplite® Colorimetric Glucose Oxidase Assay Kit

Amplite® Colorimetric Glucose Oxidase Assay Kit

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.

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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.

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Pierce™ Cypridina Luciferase Glow Assay Kit

Pierce™ Cypridina Luciferase Glow Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.

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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.

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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Supplier: AAT BIOQUEST

Lead is a highly poisonous metal to human beings, especially young children.

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Gaussia luciferase glow assay kit, Pierce™

Gaussia luciferase glow assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.

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