You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
MMP-3 Green™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Pierce™ Gaussia Luciferase Flash Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Amplite® Colorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.
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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Amplite® Red Peroxidase Substrate
Supplier: AAT BIOQUEST
Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Ethanol Quantitation Kit
Supplier: AAT BIOQUEST
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit
Supplier: AAT BIOQUEST
E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric L-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
Supplier: AAT BIOQUEST
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Colorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Total Sulfide Quantification Kit
Supplier: AAT BIOQUEST
Hydrogen sulfide is not usually a health risk at concentrations present in household water.
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Amplite® Fluorimetric Glucose Quantitation Kit
Supplier: AAT BIOQUEST
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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Amplite® Colorimetric Tyrosinase Assay Kit
Supplier: AAT BIOQUEST
Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.