You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Cypridina luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT Bioquest
Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Quantitative peroxide assay kit (lipid), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Fluorimetric Zinc Ion Quantitation Kit
Supplier: AAT Bioquest
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Glucose and Sucrose Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT Bioquest
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*
Supplier: AAT Bioquest
Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.
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Amplite® Colorimetric Biotin Quantitation Kit
Supplier: AAT Bioquest
The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.
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Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT Bioquest
Malondialdehyde (MDA) is natural byproduct of lipid peroxidation and is widely used as a key indicator to determine the oxidative stress and free radical formation.
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Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated APC proteins using a proprietary sensor, Maleimide 555™, which has an absorption peak at ~555 nm.
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β-Galactosidase Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
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Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit
Supplier: AAT Bioquest
Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.
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Amplite® Fluorimetric Melanin Assay Kit
Supplier: AAT Bioquest
Melanins have very diverse roles and functions in various organisms.
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Amplite® Luminometric Nitroreductase Assay Kit
Supplier: AAT Bioquest
Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.
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Amplite® Colorimetric D-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Ascorbic Acid Assay Kit
Supplier: AAT Bioquest
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.
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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
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Phosphatase assay kit
Supplier: G-Biosciences
The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
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Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*
Supplier: AAT Bioquest
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules.
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Amplite® Colorimetric Aldehyde Quantitation Kit
Supplier: AAT Bioquest
Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance.
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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit
Supplier: AAT Bioquest
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.