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225 results for Enzyme Assays

You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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ONPG assay kit

ONPG assay kit

Supplier: OZ BIOSCIENCES

ONPG assay kit is ideal to quantify high expression level of beta-Gal.

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Amplite® Fluorimetric Pyruvate Assay Kit

Amplite® Fluorimetric Pyruvate Assay Kit

Supplier: AAT Bioquest

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.

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Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Supplier: AAT Bioquest

Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.

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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.

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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.

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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Supplier: AAT Bioquest

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.

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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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MMP-3 Green™ Substrate

MMP-3 Green™ Substrate

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Colorimetric Asparaginase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.

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Pierce™ Gaussia Luciferase Flash Assay Kit

Pierce™ Gaussia Luciferase Flash Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.

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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Supplier: AAT Bioquest

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.

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Amplite® Red Peroxidase Substrate

Amplite® Red Peroxidase Substrate

Supplier: AAT Bioquest

Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.

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Amplite® Ethanol Quantitation Kit

Amplite® Ethanol Quantitation Kit

Supplier: AAT Bioquest

The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.

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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Supplier: AAT Bioquest

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

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Peroxide assay kit, PEROXsay™

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Supplier: AAT Bioquest

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit

Supplier: AAT Bioquest

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Fluorimetric Calcium Quantitation Kit  *Red Fluorescence*

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*

Supplier: AAT Bioquest

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric Glycerol Assay Kit

Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT Bioquest

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT Bioquest

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT Bioquest

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT Bioquest

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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