Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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Fluorescent nerve terminal probes and stains
Supplier: Biotium
Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.
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CF® Dye Dextran 150000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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Annexin V CF® dye conjugates, azide-free, lyophilised
Supplier: Biotium
Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.
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Potassium assay kits, FLIPR®
Supplier: Molecular Devices
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium
Supplier: Biotium
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
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Uric acid assay kit
Supplier: Cayman Chemical
Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.
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Prostaglandin Screening Library I
Supplier: Cayman Chemical
For screening of PGF analogs.
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Solutions colorantes, Ghost Dye™
Supplier: Tonbo Biosciences
Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.
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ViaFluor® 647 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with far-red fluorescence.
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ViaFluor® 405 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with blue fluorescence.
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Calcium assay kits, Fura-2 QBT™ Calcium kit
Supplier: Molecular Devices
The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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