You searched for: Stains
Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.
Epoxy embedding medium, hardener MNA, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator
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Embedding kit JB-4, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.
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Xylene substitute, Neo-Clear®, Sigma-Aldrich®
Supplier: Merck
Neo-Clear® is a non hazardous alternative to xylene. It is virtually odourless, compatible with most high purity solvents and is characterised by lower evaporation rates than xylene, making it a lower health risk.
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VWR® Gurr™ Histological Fixatives, AFA
Supplier: VWR Chemicals
Acidified alcoholic formalin for the fixation of glycogen.
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Pierce™ Silver Stain Rescue Reagent
Supplier: Thermo Fisher Scientific
Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.
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GelCode™ Blue Safe Protein Stains
Supplier: Thermo Fisher Scientific
GelCode blue safe protein stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.
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Aqueous mounting medium, Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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VWR® HistoChoice®, Clearing agent
Supplier: VWR Chemicals
HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.
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Pierce™ Glycoprotein Staining Kit
Supplier: Thermo Fisher Scientific
The Pierce Glycoprotein Staining Kit is a convenient, fast, and sensitive colorimetric kit for staining glycosylated proteins in polyacrylamide gels or nitrocellulose membranes using the periodic acid-Schiff (PAS) method.
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VWR® Q path® Safesolv, Solvent Substitute
Supplier: VWR Chemicals
Q Path® Safesolv is a non hazardous replacement for xylene or toluene. It is compatible with the Q Path® Safemount mounting medium reference 00647520. It is used in place of xylene, toluene, methylecyclohexane or other solvents in the course of the dehydration stages of histological specimens and during the stages of staining and lamella mounting.
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VWR® Bouin's solution
Supplier: VWR Chemicals
VWR® Bouin tissue fixative solution.
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VWR® Fixing Solution
Supplier: VWR Chemicals
Fixing solution with 0,02% formaldehyde in methanol 50% (v/v)).
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Aqueous mounting medium, glycerol gelatin, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Glycerol alters the hygroscopic property of gelatin and its permeability to water vapour. Glycerol gelatin or glycerine jelly medium is an aqueous slide mounting media, used for mounting samples in microscopy and histology.
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Decalcifier solutions, Osteomoll® and Osteosoft®, Sigma-Aldrich®
Supplier: Merck
Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.
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Pierce™ Zinc Reversible Stain Kit
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce zinc reversible stain kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.
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VWR® Cryolab Q Path, Freezing Aerosol
Supplier: VWR Chemicals
Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.
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Mounting medium, Entellan® new
Supplier: Merck
Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.
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Spurr low viscosity embedding kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.
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VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy
Supplier: VWR Chemicals
High viscosity mounting media for cryotomy. Less viscous than O.C.T.
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Gelite™ Safe DNA Gel Stain *GelRed Replacement, 10000X in DMSO*
Supplier: AAT BIOQUEST
Gelite™ Safe has been developed specifically to be less hazardous than EtBr for staining DNA in agarose and acrylamide gels with much higher sensitivity.
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Gelite™ Safe DNA Gel Stain *GelRed Alternative, 10000X in DMSO*
Supplier: AAT BIOQUEST
Gelite™ Safe has been developed specifically to be less hazardous than EtBr for staining DNA in agarose and acrylamide gels with much higher sensitivity.
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Embedding and mounting media
Supplier: Merck
The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.
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VWR® Q Path® Stick on (Cell adhesive) for Microscopy
Supplier: VWR Chemicals
Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.
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VWR® Q Path® Easyfix, Cytology Fixative
Supplier: VWR Chemicals
Cytology fixative specially designed for the conservation and transport of cytology specimens.
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J.T.Baker®, Mounting Medium, High, Histo Grade
Supplier: Avantor
Mounting medium for coverslipping of slides.
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Immersion oil, BioChemica
Supplier: PanReac AppliChem
Biochemical grade immersion oil for microscopy.
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Water-free mounting medium, Neo-Mount®
Supplier: Merck
Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.
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VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade
Supplier: VWR Chemicals
Decalcifier according to Kristenson.
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VWR® Q Path® DC3, Decalcifier Rapid-Acting
Supplier: VWR Chemicals
Contains a mixture of hydrochloric acid and alcohols with some EDTA.
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Mounting medium, kaiser's glycerol gelatine, phenol-free
Supplier: Merck
Kaiser's glycerol gelatin is an aqueous mounting medium for microscopy and works well with specimens prepared for enzyme, lipid, and immunohistochemistry determinations.