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325 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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VWR® Antibody signal enhancer

VWR® Antibody signal enhancer

Supplier: VWR Chemicals

Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.

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SeramunBlock

Supplier: Apollo Scientific

Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.

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Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Supplier: Biotium

Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

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femtoCHROMO™- AP Substrate for chromogenic detection

femtoCHROMO™- AP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

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CF™ dye bovine serum albumin conjugates

Supplier: Biotium

Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.

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CF® Dye cAMP

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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Antibody labelling kits, Mix-n-Stain™ FITC

Antibody labelling kits, Mix-n-Stain™ FITC

Supplier: Biotium

Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

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HRP-conjugate stabiliser, SeramunStab® STA2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

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ExoBrite™ True EV Membrane Stains

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

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Reactive quencher dyes

Supplier: Biotium

Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Biotin kits, Micro HOOK™

Supplier: G-Biosciences

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

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B-Phycoerythrin (BPE)

B-Phycoerythrin (BPE)

Supplier: Columbia Biosciences

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

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Dye conjugates dUTP CF®

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Biotin SE protein labelling kit

Supplier: Biotium

This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.

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HRP-conjugate stabiliser, SeramunStab® STNP

Supplier: Apollo Scientific

Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.

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Bilirubin conjugate, sodium salt

Supplier: Cayman Chemical

Bilirubin conjugate is a stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives.

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Calmodulin Resin Kit for CBP purification

Calmodulin Resin Kit for CBP purification

Supplier: G-Biosciences

The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.

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ExoBrite™ CTB EV Staining Kits, Biotium

Supplier: Biotium

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

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Reactive oxygen species indicators dyes

Supplier: Biotium

Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

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Amine reactive biotin reagents, HOOK™

Supplier: G-Biosciences

HOOK™ NHS-Biotin, 50 mg

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Reactive biotin compounds

Supplier: Biotium

Biotium offers reactive biotin compounds with a variety of reactive functional groups.

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Antibody labelling kits, Mix-n-Stain™ Biotin

Antibody labelling kits, Mix-n-Stain™ Biotin

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

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Annexin V conjugates

Annexin V conjugates

Supplier: Biotium

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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